VemoZyme® F NTP is an intrinsically thermostable phytase enzyme preparation with activity of min 5000 phytase units per gramme (for VemoZyme® F 5000 NTP) and min 10000 phytase units per gramme for VemoZyme® F 10000 NTP). One phytase unit corresponds to the quantity enzyme preparation, needed to release 1 μmol inorganic phosphorus from sodium phytate per 1 minute at temperature 37oC and pH 5.0.

 

Enzyme Characteristics 


VemoZyme® F NTP is naturally thermostable and keeps stable activity during storing, homogenizing and granulating up to 90-95oC. The product shows highly efficient enzyme activity in a wide range of pH from 2.0 to 6.0.
 

Table 1. Enzyme activity conservation rate of VemoZyme® F NTP, at 3 min, under different temperature

VemoZyme® F NTP is a new generation intrinsically thermostable phytase ezyme, which provides for its great advantage over the major coated thermostable phytases. It is a well-known fact that the additional coating of the most thermostable phytase products decreases their performance in the gut because of the highly extended dissolution time, which considerably reduces the utilization and efficacy of the enzymes. Therefore the appropriate dissolution time is an essential characteristic for the efficient use of phytase and the best way to achieve it, is by applying a naturally thermostable phytase (non-coated), which ensures a prolonged gut activity without the undesirable side effects of the coating.

VemoZyme® F NTP works best in acidic environment and is quite resistant to the digestive enzymes pepsin and pancreatin. This is the reason why it is transferred further in the gastrointestinal tract in an intact and more active form, compared to other phytases.

VemoZyme® F NTP residual phytase activity in different segments of digestive tract of chickens, compared to other commercial phytases

Residual phytase activity under inactivation with pepsin and pancreatin

 

Application


About 60 - 85% of the total phosphorus in cereals, pulses and oilseed crops cannot be assimilated by non-ruminants, because it exists in the form of phosphorus phytate.

The phosphoric groups in phytic acid are electronegative and have very strong chelating capacity with some cations such as Ca2+, Mg2+, Zn2+, Cu2+, Mn2+, Fe2+, K+ etc., thus influencing the absorption and utilization of these minerals by livestock.

The phosphoric groups in phytic acid can also combine with  cation groups on proteins, amino acids, starch and lipids in feed and reduce their solubility, thus influencing the digestibility of these nutritional materials by livestock.

Phytic acid can also combine with enzymes in the animal body such as amylase, pepsin, trypsin, acid phosphatase, etc. It reduces the activity of these enzymes, resulting in a decreased utilization rate of ration nutrients.

Phytate is a major anti-nutrition factor in the feed of monogastric species. It can reduce the utilization rate of feed phosphorus and other nutrients by animals.

The utilization of phosphorus from phytate becomes possible with the addition of phytase enzyme, because animals do not produce this enzyme themselves. The application of phytase is a well known method for degrading phytate complexes and utilizing the phytate phosphorus from plants.
 

Biological Action


The use of phytase has great biological, economical and ecological benefits:
♦    Increases phosphorus phytate utilization rate of feed by 40%~60%
♦    Increases the utilization rate of calcium, zinc, copper, magnesium and other minerals
♦    Increases the utilization rate of proteins, amino acids, starch and lipids in the feed
♦    Decreases the addition of inorganic phosphorus with 6-8 kg per ton feed
♦    Decreases phosphorus content in the animal's excrements and pollution of the environment
 

Dosage


VemoZyme® F 5000 NTP:   
♦    100-150 g/t feed for pigs and poultry (500 - 750 U/kg) 
♦    60-90 g/t feed for laying hens (300 - 450 U/kg)

VemoZyme® F 10000 NTP: 
♦    50-75 g/t feed for pigs and poultry (500 - 750 U/kg) 
♦    30-45 g/t feed for laying hens (300 - 450 U/kg)
 

Storage 


In dry and cool places away from direct sunlight at a temperature under 25° С
 

Experimental Data

VemoZyme® F NTP in poultry feed

VemoZyme® F NTP in pig feed

Experimental design
Positive Control (PC): without phytase
Negative Control (NC): reduced AP compared with positive control
NC + VemoZyme® F NTP: with addition of
VemoZyme® F NTP to NC diet

Experimental design:
Positive Control (PC): without phytase
Negative Control (NC): reduced AP compared with positive control
NC + VemoZyme® F NTP: with addition of
VemoZyme® F NTP to NC diet

By increasing the utilization of phosphorus and nutrients in the compound feed, VemoZyme® F NTP improves production performance and at the same time helps protect the environment.

 

Experimental design
Positive Control (PC): Broilers fed with normal P/normal Ca corn-soybean meal diet
Negative Control (NC): Broilers fed with low P/low Ca corn-soybean meal diet

VemoZyme® F NTP in broilers feed

Conclusion
VemoZyme® F NTP
addition is shown to have a positive effect on crude protein, essential amino acids, and non-essential amino acids digestibility. This improvement is observed to be considerable in both groups supplemented with VemoZyme® F NTP, with the results being close to or even better than the PC.